Department of Biochemistry and Medical Genetics, Touro College of Osteopathic Medicine, Middletown, NY 10940, USAUnited States
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Clinical biochemistry and pathology have contributed too many assays for diagnosis and prognosis of human health and diseases. Bedside biochemistry has revolutionized modern medicine and the invention of new generation biosensors have educated the patients like never before. Although, there are many gadgets which are available for monitoring the blood glucose in the patients with metabolic syndromes, hardly any assays can accurately predict and aid in early detection of metabolic syndrome. By the time a person is diagnosed and treated for metabolic syndrome, molecular and pathological damages further push the patient towards the metabolic syndrome. Early detection and early treatments are necessary to further revolutionize the field of Medicine.
CANCER STUDIES AND MOLECULAR MEDICINE ISSN 2377-1518 http://dx.doi.org/10.17140/CSMMOJ-3-118 Open Journal Letter to the Editor Is it Time to Start Using Mitochondrial DNA * *Corresponding author Copy Number as an Indicator of Health Vasudeva G. Kamath, MSc, PhD Assistant Professor Department of Biochemistry and and Diseases?
Medical Genetics Touro College of Osteopathic Medicine 60 Prospect Avenue * Vasudeva G. Kamath, MSc, PhD Middletown, NY 10940, USA Tel. 845-648-1250 E-mail: vasudeva.kamath@touro.edu Department of Biochemistry and Medical Genetics, Touro College of Osteopathic Medicine, Middletown, NY 10940, USA Volume 3 : Issue 2 Article Ref. #: 1000CSMMOJ3118 Clinical biochemistry and pathology have contributed too many assays for diagnosis and prognosis of human health and diseases. Bedside biochemistry has revolutionized modern Article History medicine and the invention of new generation biosensors have educated the patients like never th Received: May 19th, 2017 before. Although, there are many gadgets which are available for monitoring the blood glucose th Accepted: May 25th, 2017 in the patients with metabolic syndromes, hardly any assays can accurately predict and aid th Published: May 25th, 2017 in early detection of metabolic syndrome. By the time a person is diagnosed and treated for metabolic syndrome, molecular and pathological damages further push the patient towards the Citation metabolic syndrome. Early detection and early treatments are necessary to further revolutionize Kamath VG. Is it time to start us- the field of Medicine.
ing mitochondrial DNA copy num- ber as an indicator of health Metabolic syndrome is not a completely solved riddle, there are several factors which and diseases? Cancer Stud Mol Med Open J. 2017; 3(2): 21-22. contribute towards the genesis and progression of the disease. Energy metabolism is in the doi: 10.17140/CSMMOJ-3-118 center of metabolic syndrome, and mitochondria are one such organelle where majority of the energy metabolism takes place. Given that it is at the center of energy metabolism, most of the proteins required for this process are supplied by the nuclear gene products. Additionally, the mitochondria with its genome is responsible for 13 of the key electron transport com- plex proteins. Mutations in both nuclear and mitochondrial genes result in Mitochondrial En- cephalomyopathy Lactic Acidosis and Stroke-like Episodes (MELAS), Myoclonic Epilepsy and Ragged-Red Fiber Disease (MERF), Mitochondrial Neurogastrointestinal Encephalopathy (MNGIE) and Leigh syndrome.
The mitochondrial genome is a simple closed circular molecule that contains 16,569 DNA base pairs compared to the complex nuclear genome. But on the other hand, the nuclear genome has specified amount i.e., two copies at the least of every gene and the quality of the ge- nome is well maintained by “well-oiled” DNA repair machinery. In mitochondrial genome con- tains numerous copies of the genes. Apart from this the number of mitochondria in each tissue also varies. This imbalance in quantity of proteins generated from mitochondria and nucleus for electron transport complex is a testimony of their importance in energy metabolism. Mitochon- drial DNA (mtDNA) quantity and the quality is an important aspect of the energy metabolism. The factors attributing towards determining the number of mitochondria and mitochondrial DNA copy number is yet unclear. Nonetheless, recent investigations suggest a negative correla- tion between mitochondrial DNA copy number and disease process and progression. A recent article Reznik et al1 clearly demonstrated the correlation between mtDNA copy number to the expression of mitochondrially-localized metabolic pathways, suggesting that mtDNA copy number variation reflects gross changes in mitochondrial metabolic activity. As we discussed Copyright the significance of the mitochondrial DNA copy number, it is also important to note the qual- ©2017 Kamath VG. This is an open access article distributed under the ity of the DNA, it is observed that over a period of time the bad copies of the mitochondrial Creative Commons Attribution 4.0 DNA accumulate within tissue mitochondria as the mitochondrial DNA repair mechanism is International License (CC BY 4.0), not as effective as the nuclear DNA repair mechanisms and the chances of mutation at the end which permits unrestricted use, dis- of every replication cycle is higher. With this delicate balance of the quality and quantity of the tribution, and reproduction in any medium, provided the original work mtDNA determining the efficacy of the energy metabolism, there is a high possibility that these is properly cited. changes can be used as indicators of the metabolic problems to occur in near future. It is up to ISSN 2377-1518 http://dx.doi.org/10.17140/CSMMOJ-3-118 the scientific communities to decide whether it is time for us to seriously investigate the numerical relation of mitochondrial DNA copy number as a health indicator. Appropriate detection of mtDNA mutations and quantification of mtDNA can be a very important means in the resources of the modern day physicians.