Influence of Body Size on Blood Hemogram in Rainbow Trout Oncorhynchus Mykiss (Walbaum, 1792)

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Authors

  • Francesco Fazio Department of Veterinary Sciences, University of Messina, Polo Universitario dell’ Annunziata, 98168, Messina, Italy
  • Concetta Saoca Department of Veterinary Sciences, University of Messina, Polo Universitario dell’ Annunziata, 98168, Messina, Italy
  • Irene Vazzana Department of Veterinary Sciences, University of Messina, Polo Universitario dell’ Annunziata, 98168, Messina, Italy
  • Giuseppe Piccione Department of Veterinary Sciences, University of Messina, Polo Universitario dell’ Annunziata, 98168, Messina, Italy

DOI:

https://doi.org/10.17140/VMOJ-2-121

Keywords:

Body size; Biometric indices; Hematological parameters; Trout.

Abstract

Aim: The aim of this study was to investigate the influence of body size on hematological parameters in Rainbow trout Oncorhynchus mykiss (Walbaum, 1792).
Materials and Methods: For the purpose of the study, fifty Italian trout were divided into two
groups: Group S1 (n=25) of weight (300-500 g) and length (25-32 cm); Group S2 (n=25) of
weight (510-700 g) and length (33-38 cm). Blood samples were collected from each fish, to
record the count of white blood cell (WBC), red blood cell (RBC), determine the hemoglobin
concentration (Hb), hematocrit (Hct) value, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) mean corpuscular hemoglobin concentration (MCHC), and thrombocyte count (TC).
Results: The collected blood was analyzed using an automatic method to determine the hematological parameters. Statistical analysis (unpaired t-test) showed a significant effect of size
(p<0.05) on the RBC count, Hb concentration and Hct value. No statistically significant differences were observed in the WBC count, MCV, MCH concentration, MCHC and TC.
Conclusion: The data reported in this study contributed towards the knowledge of hematological evaluation in farmed trout and showed that fish size was an important factor that helped
establish the “physiological range” supporting the accurate interpretation of hematological parameters in examining the health status of this species.

References

Associazione Piscicoltori Italiani. 2005. La piscicoltura Italiana. Web site. http://www.api-online.it. 2004.

Yavuscan YH, Bekcam S, Karasu Benli AC, Akan M. Some blood parameters in the eel (Anguilla Anguilla) spontaneously infected with Aeromonas hydrophila. Aquacult Res. 2005; 60: 1388-1390.

Nilza LR, Ligia MM, Dense OS, et al. Haematological and biochemical values for Nile tilapia (Orechromis niloticus) cultured in semi-intensive system. Acta Sci Biol Sci Maringa. 2003; 25: 385-389.

Adam HM, Agab H. Haematological and biochemical indices of Clarias garipeinus collected from River Nile, Sudan. Sudan Acad Sci J. 2007; 21: 67-90.

Caruso G, Denaro MG, Caruso R, Mancari F, Genovese L, Maricchiolo G. Response to short term starvation of growth, haematological, biochemical and non-specific immune parameters in European sea bass (Dicentrarchus labrax) and blackspot sea bream (Pagellus Bogaraveo). Mar Environ Res. 2011; 72(1-2): 46-52. doi: 10.1016/j.marenvres.2011.04.005

Ighwela KA, Ahmad AB, Abol-Munafi AB. Haematological changes in nile tilapia (Oreochromis niloticus) fed with varying dietary maltose levels. World J Fish Mar Sci. 2012; 4(4): 376- 381.

Faggio C, Casella S, Arfuso F, Marafioti S, Piccione G, Fazio F. Effect of storage time on haematological parameters in mullet, Mugil cephalus. Cell Biochem Funct. 2013; 31(5): 412-416. doi: 10.1002/cbf.2915

Fazio F, Saoca C, Casella S, Fortino G, Piccione G. Relationship between blood parameters and biometric indices of Sparus aurata and Dicentrarchus labrax cultured in onshore tanks. Mar Freshw Behav Physiol. 2015; 48: 289-296. doi: 10.1080/10236244.2015.1041239

Jawad LA, Al-Mukhtar MA, Ahmed HK. The relationship between haematocrit and some biological parameters of the Indian shad, Tenualosa ilisha (Family Clupeidae). Animal Biodivers Conserv. 2004; 27: 47-52.

Fazio F, Faggio C, Marafioti S, Torre A, Sanfilippo M, Piccione G. Effect of water quality on hematological and biochemical parameters of Gobius niger caught in faro lake. (SICILY). Iranian J Fish Sci. 2013; 12(1): 219-231.

Raizada MN, Jain KK, Raizada S. Monthly variations in the hematocrit values (PCV) in a teleost, Cirrhinus mrigala (Ham.). J Comp Anim Physiol. 1983; 8: 196-198.

Chaudhuri SH, Pandit T, Benerjee S. Size and sex related variations of some blood parameters of Sarotheriodon mossambica. Environ Ecol. 1986; 4(1): 61-63.

Tran-Duy A, Schrama JW, Van Dam AA, Verreth JAJ. Effects of oxygen concentration and body weight on maximum feed intake, growth and haematological parameters of anile tilapia, Oreochromis niloticus. Aquaculture. 2008; 275(1-4): 152-162. doi: 10.1016/j.aquaculture.2007.12.024

Ranzani-Paiva MJT. Caracteristicas hematológicas de tainha Mugil platanus Günther, 1880 (Osteichthyes, Mugilidae) da regiao estuarino-lagunar de Cananeia-SP (lat. 25 °00’S-long. 47 °55’W). Bol Inst Pesca. 1995; 22: 1-22.

Ranzani-Paiva MJT, Rodrigues EDL, Veiga MLD, Eiras ACD. Association between the haematological characteristics and the biology of the “dourado” Salminus Maxillosus Valenciennes, 1840 from Mogi-Guaçu River, state of São Paulo, Brazil. Maring. 2001; 23: 527-533.

Luskova V. Annual cycles and normal values of hematological parameters in fishes. Acta Sc Nat Brno. 1997; 31(5): 70.

Modrá H, Svobodová Z, Kolářová J. Comparison of differential leukocyte counts in fish of economic and indicator importance. Acta Vet Brno. 1998; 67(4): 215-226.

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Published

2017-11-30

How to Cite

Francesco Fazio, Concetta Saoca, Irene Vazzana, & Giuseppe Piccione. (2017). Influence of Body Size on Blood Hemogram in Rainbow Trout Oncorhynchus Mykiss (Walbaum, 1792). Veterinary  Medicine, 2(3), 91–94. https://doi.org/10.17140/VMOJ-2-121

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