Analysis of Antibiotic Resistance of the Probiotic Bacteria Found in Commercial Food Products
DOI:
https://doi.org/10.17140/VROJ-4-115Keywords:
Lactobacillus, Probiotics, Antibiotics, Tetracycline, Vancomycin, Erythromycin, Ampicillin, Antibiotic resistanceAbstract
Aim
The Lactobacillus is an industrially-important group of probiotic organisms that plays an important role in human health by inhibiting
harmful and pathogenic bacterial growth, boosting immune function, and increasing resistance to infection. The aim of this study
was to identify the probiotic bacteria Lactobacillus based on their phenotypic features and genotypic features. This study also shows
the importance of probiotic bacterium, and the effects of their antibiotic resistance to human.
Method
Six different brands were cultured on man, rogosa and sharpe (MRS) agar. The identity of the culture was based on the
characteristics of the strains of Lactobacillus spp. which was characterized using their phenotypic features (cell morphology, Gram’s
staining tests which are specific for Lactobacillus genus). The bacterial deoxyribonucleic acid (DNA) was extracted by two different
methods, boiled cell method and cetyl trimethylammonium bromide (CTAB) method. Furthermore, the extracted DNA yields
were compared to determine which gives the best yield. The bacterial genus was detected with using genus specific primers, specific
to the Lactobacillus. All the isolates were further subjected to antibiotic resistance test using disc diffusion method against a total of
4 antibiotics (Erythromycin, Tetracycline, Vancomycin and Ampicillin) and the antibiotic resistant genes of tet(M) & erm(B), were
analyzed by polymerase chain reaction (PCR).
Results
Five isolates out of six samples (A to E) were found to exhibit multiple resistance against some of the most commonly used
antibiotics. The isolates showed resistance toward tetracycline, erythromycin & vancomycin. Besides that, the isolates displayed a
low-level of resistance toward ampicillin.
Conclusion
This study proves that antibiotic resistance is present in different species of probiotic strains, which may pose a food safety concern.
References
Martín R, Miquel S, Ulmer J, Kechaou N, Langella P, Bermúdez-Humarán LG. Role of commensal and probiotic bacteria in human health: A focus on inflammatory bowel disease. Microb Cell Fact. 2013; 12: 71. doi: 10.1186/1475-2859-12-71
Farnworth ER. The evidence to support health claims for pro-biotics. J Nutr. 2008; 138: 1250-1254. doi: 10.1093/jn/138.6.1250S
Shah N. Probiotic bacteria: Selective enumeration and survival in dairy foods. J Dairy Sci. 2000; 83: 894-907. doi: 10.3168/jds.S0022- 0302(00)74953-8
Saarela M, Mogensen G, Fonden R, Matto J, Mattila-Sandholm T. Probiotic bacteria: Safety, functional and technological properties. J Biotechnol. 2000; 84: 197-215. doi: 10.1016/s0168-1656(00)00375-8
Zheng M, Zhang R, Tian X, Zhou X, Pan X, Wong A. Assess-ing the risk of probiotic dietary supplements in the context of antibiotic resistance. Front Microbiol. 2017; 8: 1-8. doi: 10.3389/ fmicb.2017.00908
Nagpal R, Kumar A, Kumar M, Behare P, Jain S, Yadav H. Probiotics, their health benefits and applications for developing healthier foods: A review. FEMS Microbiol Lett. 2012; 334: 1-15. doi: 10.1111/j.1574-6968.2012.02593.x
Ouwehand A, Forssten S, Hibberd A, Lyra A, Stahl B. Probiotic approach to prevent antibiotic resistance. Ann Med. 2016; 48: 246- 255. doi: 10.3109/07853890.2016.1161232
Imperial I, Ibana J. Addressing the antibiotic resistance problem with probiotics: Reducing the risk of its double-edged sword ef-fect. Front Microbiol. 2016; 7: 1983. doi: 10.3389/fmicb.2016.01983
Gueimonde M, Sanchez B, G de los Reyes-Gavilan C, Margolles A. Antibiotic resistance in probiotic bacteria. Front Microbiol. 2013; 4: 202. doi: 10.3389/fmicb.2013.00202
Amara A, Shibl A. Role of probiotics in health improvement, infection control and disease treatment and management. Saudi Pharm J. 2015; 23: 107-114. doi: 10.1016/j.jsps.2013.07.001
Kale PS. Isolation and identification of bacteria from curd and its application in probiotic chocolate. Eur J Experiment Biol. 2014; 4: 95-97.
Goldstein E, Tyrrell K, Citron D. Lactobacillus species: Taxo-Submit your article to this journal | https://openventio.org/submit-manuscript/ nomic complexity and controversial susceptibilities. Clin Infect Dis. 2015; 60: 98-107. doi: 10.1093/cid/civ072
Teixeira P. Encyclopedia of Food Microbiology. 1st ed. New York, USA: Academic Press; 1999: 1136-1144.
Teixeira P. Encyclopedia of Food Microbiology. 2nd ed. New York, USA: Academic Press; 2014; 425-431.
Mitra A, Ghoshal S. Growth and survival of pathogenic E. coli during curdling of milk. International Journal of Life Sciences Biotechnol-ogy and Pharma Research. 2014; 3: 330-331.
Hervert C, Alles A, Martin N, Boor K, Wiedmann M. Evalu-ation of different methods to detect microbial hygiene indicators relevant in the dairy industry. J Dairy Sci. 2016; 99: 7033-7042. doi: 10.3168/jds.2016-11074
Gad G, Abdel-Hamid A, Farag Z. Antibiotic resistance in lactic acid bacteria isolated from some pharmaceutical and dairy products. Braz J Microbiol. 2014; 45: 25-33. doi: 10.1590/s1517-
Abu Bakar F, Abdulamir A, Nordin N, Yoke T. Methods for precise molecular detection of probiotic microflora: Using adjusted molecular biology protocols, primer sets and PCR assays. Biotech-nology (Faisalabad). 2010; 9: 25-32. doi: 10.3923/biotech.2010.25.32
Sharma P, Tomar S, Sangwan V, Goswami P, Singh R. Antibiotic resistance of lactobacillussp. Isolated from Commercial Probiotic Preparations. J Food Safety. 2015; 36: 38-51. doi: 10.1111/jfs.12211
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