Efficacy of Natural Herbal Formulation against Acute Hepatopancreatic Necrosis Disease (AHPND) causing Vibrio parahaemolyticus in Penaeus vannamei

Abstract

A formulation was developed using combination of blended natural essential oils as an antiVibrio parahemolyticus causing acute hepatopancreatic necrosis disease (AHPND) candidate.
Lavandula latifolia, Pinus sylvestris, Jasminum officinale, Citrus limon, Prunus avium, Viola
odorata, Gardenia jasminoides, Cocos nucifera, Rosa damascene and Eucalyptus globulus,
mixed together to develop as anti-V. parahemolyticus product. The treatment group was fed
on essential oil mixed feed whereas control group were fed on the regular feed throughout the
experiment. The shrimp of both treatment and control were challenged by immersion method
at day 8. The cumulative AHPND-gross sign appearance in positive control reached up to 95%
at dpi 10 whereas no gross sign appeared in treatment and in negative control. The cumulative
mortality reached up to 46.7% at dpi 10 in positive controls whereas no mortality recorded in
treatment and in negative control. The V. parahaemolyticus isolated from the hepatopancreas
of infected shrimp matched 100% with the existing AHPND strain. The trial results show that
the developed natural herbal formulation has significant effect against AHPND in a controlled
condition