Efficacy of Natural Herbal Formulation against Acute Hepatopancreatic Necrosis Disease (AHPND) causing Vibrio parahaemolyticus in Penaeus vannamei
Keywords:
Acute hepatopancreatic necrosis disease (AHPND); Vibrio parahemolyticus; Essential oil blend; anti-AHPND feed.Abstract
A formulation was developed using combination of blended natural essential oils as an antiVibrio parahemolyticus causing acute hepatopancreatic necrosis disease (AHPND) candidate.
Lavandula latifolia, Pinus sylvestris, Jasminum officinale, Citrus limon, Prunus avium, Viola
odorata, Gardenia jasminoides, Cocos nucifera, Rosa damascene and Eucalyptus globulus,
mixed together to develop as anti-V. parahemolyticus product. The treatment group was fed
on essential oil mixed feed whereas control group were fed on the regular feed throughout the
experiment. The shrimp of both treatment and control were challenged by immersion method
at day 8. The cumulative AHPND-gross sign appearance in positive control reached up to 95%
at dpi 10 whereas no gross sign appeared in treatment and in negative control. The cumulative
mortality reached up to 46.7% at dpi 10 in positive controls whereas no mortality recorded in
treatment and in negative control. The V. parahaemolyticus isolated from the hepatopancreas
of infected shrimp matched 100% with the existing AHPND strain. The trial results show that
the developed natural herbal formulation has significant effect against AHPND in a controlled
condition
References
Osborne CA. Three steps to effective management of bac-terial urinary tract infections: Diagnosis, diagnosis, diagnosis. Compendium. 1995; 17: 1233-1240. Web site. http://agris.fao. org/agris-search/search.do?recordID=US9629922. Accessed December 13, 2016.
Tilley LP, Smith Jr. FWK. The 5-Minute Veterinary Consult: Canine and Feline. 3rd ed. Philadelphia, Pennsylvania, USA: Lippincott Williams & Wilkins; 2004: 768-769.
Kruth S. Urinary tract infections. In: Giguère S, Prescott JF, Baggot JD, Walker RD, Dowling PM, eds. Antimicrobial Thera-py in Veterinary Medicine. 4th ed. Oxford, England, UK: Black-well Publishing; 2006: 372-379.
Bartges JW. Diseases of the urinary bladder. In: Birchard SJ, Sherding RG, eds. Saunders Manual of Small Animal Practice. 2nd ed. Philadelphia, Pennsylvania, USA: WB Saunders Co; 2000: 943-957.
Cohn LA, Gary AT, Fales WH, Madsen RW. Trends in fluo-roquinolone resistance of bacteria isolated from canine urinary tracts. J Vet Diagn Invest. 2003; 15: 338-343. Web site. https:// www.ncbi.nlm.nih.gov/pubmed/12918814. Accessed December 13, 2016.
Hall JL, Holmes MA, Baines SJ. Prevalence and antimicro-bial resistance of canine urinary tract pathogens. Vet Rec. 2013; 173: 549. doi: 10.1136/vr.101482
Windahl U, Holst BS, Nyman A, Gronlund U, Bengtsson B. Characterisation of bacterial growth and antimicrobial suscepti-bility patterns in canine urinary tract infections. BMC Vet Res. 2014; 10: 217. doi: 10.1186/s12917-014-0217-4
Wong C, Epstein SE, Westropp JL. Antimicrobial susceptibil-ity patterns in urinary tract infections in dogs (2010-2013). J Vet Intern Med. 2015; 29: 1045-1052. doi: 10.1111/jvim.13571
Marques C, Gama LT, Belas A, et al. European multicenter study on antimicrobial resistance in bacteria isolated from com-panion animal urinary tract infections. BMC Vet Res. 2016; 12: 213. doi: 10.1186,s12917-016-0840-3
Hariharan H, Brathwaite-Sylvester E, Belmar VM, Sharma R. Bacterial isolates from urinary tract infection in dogs in Gre-nada, and their antibiotic susceptibility. Open J Vet Med. 2016; 6: 85-88. doi: 10.4236/ojvm.2016.66010
Quinn PJ, Carter ME, Markey B, Carter GR. Clinical Veteri-nary Microbiology. London, England, UK: Wolf/Mosby; 1994.
National Committee for Clinical Laboratory Standards. Per-formance Standard for Antimicrobial Disk and Dilution Suscep-tibility Tests for Bacteria Isolated from Animals: Approved Stan-dard. 2nd ed. Wayne, PA, USA: NCCLS; 2002: M31-A2.
Dowling PM. Antimicrobial therapy of urinary tract infec-tions. Can Vet J. 1996; 37: 438-441. Web site. https://www.ncbi. nlm.nih.gov/pmc/articles/PMC1576458/. Accessed December 13, 2016.
McMeekin CH, Hill KE, Gibson IR, Bridges JP, Benschop J. Antimicrobial resistance patterns of bacteria isolated from canine urinary samples submitted to a New Zealand veterinary diagnostic laboratory between 2005-2012. N Z Vet J. 2016; 14: 1-6. doi: 10.1080/00480169.2016.1259594
Barsanti JA. Genitourinary infections. In: Greene CE, ed. Infectious Diseases of the Dog and Cat. 3rd ed. St. Louis, MO, USA: Saunders/Elsevier; 2006: 935-961.
Gibson JS, Morton JM, Cobbold RN, Sidjabat HE, Filip-pich LJ, Trott DJ. Multidrug-resistant E. coli and enterobacter extraintestinal infection in 37 dogs. J Vet Intern Med. 2008; 22: 844-850. doi: 10.1111/j.1939-1676.2008.00124.x
Wilson BJ, Norris JM, Malik R, et al. Susceptibility of bacte-ria from feline and canine urinary tract infections to doxycycline and tetracycline concentrations attained in urine four hours af-ter oral dosage. Aust Vet J. 2006; 84: 8-11. doi: 10.1111/j.1751- 0813.2006.tb13115.x
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