High-Throughput Next Generation Sequencing: Applications in Reproductive Diagnosis and Research

Abstract

Genes in a genome can be identified by creating a complementary DNA (cDNA) library from the pool of ribonucleic acid (RNA) transcripts. To generate a cDNA library, the RNA transcripts from a tissue or from cells are copied into more stable cDNA molecules, which are then stored into an appropriate vector to generate a collection of cDNA clones. The single pass, short 300-500 nucleotide sequences obtained from sequencing either end of the cDNA insert are called expressed sequence tags (ESTs). ESTs can be generated from the cDNA libraries obtained from the patient tissue/samples.