Chemical Composition of Fruit Peels Essential Oil from Citrus Species and their Antimicrobial Efficacy as Biopreservatives
DOI:
https://doi.org/10.17140/aftnsoj-8-177Keywords:
Citrus peel, Essential oil, Antimicrobial, Antioxidant, Food spoilage bacteria, FungusAbstract
Aim
Extraction, chemical determination and comparison of essential oil (EO) composition from the peel oil of three varieties of citrus, sweet lime (Citrus limetta, Meetha Nimbu), lemon (C. limon) and acid lime (C. aurantifolia, Kagzi Nimbu) and their antimicrobial efficacy as preservative in food spoilage was studied.
Material and Methods
The extraction from all the three types lemon peel oil was done using clevenger apparatus and the chemical constituents of lemon peel EO analyzed by GC-MS. The antagonistic activity of lemon peel EO and 50% methanolic extract from peel residue left after EO extraction was studied against common food borne pathogens like Staphylococcus aureus, S. epidermidis, Bacillus cereus, B. subtilis, Bacillus sp., Listeria monocytogenes, Micrococcus luteus, Escherichia coli, Klebsiella sp.and Pseudomonas aeruginosa by agar well diffusion assay. The test fungal species were Rhizopus sp., Rhizomucor sp., Alternaria sp., Aspergillus fumigatus, A. niger, Aspergillus sp. and Penicillium sp.
Results
The chemical constituents of lemon peel EO analyzed by GC-MS showed 22 constituents with limonene (39-92%), β-myrcene (0.08-2.57%), α-terpineol (0.3-7.3%), α- and β-pinene (0.2-25.4%) as major ingredients and 1-Octanol, cis-linalool oxide, sabinene as minor constituents. It was found that lemon peel oil exhibited wide range of antimicrobial activity against both groups of bacteria with highest inhibition of zone produced against Bacillus mycoides and B. cereus 34 mm and 28 mm respectively and against fungi Rhizopus sp. and Aspergillus sp. (34 mm and 28 mm respectively). In contrast to EO, the peel extract produced widest zone of inhibition against Staphylococcus aureus followed by S. epidermidis and B. subtilis (IZD of 15, 13 and 13 mm respectively) and in case of fungus, peel extract was most effective against Alternaria sp. and Rhizopus sp. (IZD 26 mm, 17 mm respectively).
Conclusion
These studies illustrated that antioxidant and antimicrobial activity of EO was much better than its lemon peel extract and has potential to be used as biopreservative in food spoilage.
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