The Proportion of ALDEFLUOR-Positive Cancer Stem Cells Changes with Cell Culture Density Due to the Expression of Different ALDH Isoforms

Authors

  • Lynn M. Opdenaker Department of Biological Sciences, University of Delaware, 118 Wolf Hall, Newark, DE 19716, USA; Center for Translational Cancer Research, Helen F. Graham Cancer Center and Research Institute, 4701 Ogletown-Stanton Rd, Newark, DE 19713, USA
  • Shirin R. Modarai Department of Biological Sciences, University of Delaware, 118 Wolf Hall, Newark, DE 19716, USA; Center for Translational Cancer Research, Helen F. Graham Cancer Center and Research Institute, 4701 Ogletown-Stanton Rd, Newark, DE 19713, USA
  • Bruce M. Boman Department of Biological Sciences, University of Delaware, 118 Wolf Hall, Newark, DE 19716, USA; Center for Translational Cancer Research, Helen F. Graham Cancer Center and Research Institute, 4701 Ogletown-Stanton Rd, Newark, DE 19713, USA

DOI:

https://doi.org/10.17140/CSMMOJ-2-113

Keywords:

ALDEFLUOR, Cancer stem cells, ALDH isoforms, Density

Abstract

A significant number of discrepancies exist within the literature regarding ALDEFLUOR- positive stem cell populations in cell lines. We hypothesized that these inconsistencies resulted from differences in culture conditions, particularly cell density. We cultured several colon cancer cell lines (N=8) at high and low densities and found a significant decrease in ALDEFLUOR-positive cell populations at high density. However, we found no changes in the CD166-positive stem cell population, self-renewal, or cell cycle distribution of cells cultured at different densities. Interestingly, when we sorted both ALDEFLUOR positive and negative populations from the different density cultures, we identified a significant number of Aldehyde dehydrogenase (ALDH) isoforms whose expression was decreased in ALDEFLUOR-positive stem cells cultured at high density. This novel finding suggests that multiple ALDH isoforms contribute to ALDEFLUOR activity in colon cancer stem cells and decreases in ALDEFLUOR- positive stem cells at high cell density are due to decreased expression of multiple ALDH isoforms. Thus, designing therapeutics to target ALDEFLUOR-positive cancer stem cells may require inhibition of multiple ALDH isoforms.

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Published

2015-12-09

How to Cite

Lynn M. Opdenaker, Shirin R. Modarai, & Bruce M. Boman. (2015). The Proportion of ALDEFLUOR-Positive Cancer Stem Cells Changes with Cell Culture Density Due to the Expression of Different ALDH Isoforms. Cancer Studies and Molecular Medicine , 2(2), 87–95. https://doi.org/10.17140/CSMMOJ-2-113

Issue

Vol. 2 No. 2 (2015): Volume 2 Issue 2 2015

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