In Vitro-Evaluation of the Antioxidant Properties of Moringa Oleifera and Camelia Sinensis Leaves

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Authors

  • Moses Babatunde Ayoola Department of Medical Biochemistry, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria
  • Nonso C. Ejiofor Department of Medical Biochemistry, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria
  • Ikechukwu Edwin Ezeagu Department of Medical Biochemistry, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria

DOI:

https://doi.org/10.17140/aftnsoj-5-152

Keywords:

Moringa oleifera, Camellia sinensis, Phytochemical, In vitro, Antioxidants, Oxidative stress

Abstract

Introduction

Moringa oleifera and Camellia sinensis are edible plants widely distributed in parts of South East of Nigeria. Both are used as a beverage and medicinal plant in the treatment of several ailments by alternative medical practitioners. The in vitro-antioxidant properties of both Moringa oleifera and Camelia sinensis leaves were investigated in the current study..

Method

Phytochemical analysis was carried out to evaluate the flavonoids and total phenolic contents of the plants crude extracts. Also, reducing power ability and nitric oxide scavenging activity of the plants were also determined in order to ascertain their antioxi-dant capacities to eliminate free radicals, and attenuate oxidative stress.

Results

Both plant samples demonstrated antioxidant properties. The samples were rich in flavonoid (135.14±5.20 and 208.24±14.38), and total phenolics (62.85±1.70 and 91.68±0.22) for both Moringa oleifera and Camelia sinensis respectively. The plant samples also showed nitric oxide scavenging and reducing power ability. Camelia sinensis appears to be a better antioxidant plant.

Conclusion

Moringa oleifera and Camelia sinensis contains substantial amount of antioxidant substances which could warrant their utilization in alternative medicine. However, further research works are needed to properly ascertain and harness these bioactive agents present in the plants.

References

Namita P, Mukesh R, Vijay K.J. Camellia Sinensis (Green Tea): A Review. Global Journal Pharmacology. 2012; 6 (2): 52-59

Khalafalla MM, Abdellatef E, Dafalla HM, et al. Active Principle from Moringa oleifera lam leaves effective against two leukemias and ahepatocarcinoma. African Journal of biotechnology. 2010; 9: 8467-8471

Mughal MHS, Ali G, Srivastava PS, Iqbal M. Improvement of drumstick (Moringa pterygosperma Gaertn). A unique source of food and medicine through tissue culture. Hamdard Med. 1999; 42(1): 37-42

Ruxton CH. Black tea and health. Nutrition Bulletin. 2008; 38 (3): 287-301. doi: 10.1111/j.1467-3010.2008.00691.x

Cabrere C, Artacho R, Gimenez R. Beneficial effects of green tea—A review. Journal of the American College of Nutrition. 2006; 25(2): 79-99

Betteridge DJ. What is Oxidative Stress?. Journal of Metabolism. 2000; 49 (21): 3-8

Schauer F. Oxygen regulation of nitrogen metabolism in microorganisms. Zentralbl Mikrobiol. 1988; 143(3): 195-206

Jones DP. Renal metabolism during normoxia, hypoxia, and ischemic injury. Annual Review of Physiology. 1986; 48: 33-50. doi: 10.1146/annurev.ph.48.030186.000341 Original Research | Volume 5 | Number 1| Ejiofor

Bhattacharyya N, Seth S,Tudu B, et al. Monitoring of black tea fermentation process using electronic nose. Journal of Food Engineering. 2007; 80(4): 1146-1156. doi: 10.1016/j.jfoodeng.2006.09.006

Bergendi L, Benes L, Durackova Z, Ferencik M. Chemistry, physiology and pathology of free radicals. Life Sci. 1999; 65(18- 19): 1865-1874. doi: 10.1016/S0024-3205(99)00439-7

Liu Y, Fiskum G, Schubert D. Generation of reactive oxygen species by the mitochondrial electron transport chain. J Neurochem. 2002; 80(5): 780-787. doi: 10.1046/j.0022-3042.2002.00744.x

Storey KB. Oxidative stress: Animal adaptation in nature. Braz J Med Biol Res. 1996; 29(12): 1715-1733

Percival M. Clinical nutrition insights: Antioxidants. Advanced Nutrition Publications Inc. 1998; 1-4

Manach C, Scalbert A, Morand C, Remesy C, Jimenez L. Polyphenols: food sources and bioavailability. Am J Clin Nutr. 2004; 79(5): 727-747. doi: 10.1093/ajcn/79.5.727

Segal AW. How neutrophils kill microbes. Annu Rev Immunol. 2005; 9(5): 197-223. doi: 10.1146/annurev.immunol.23.021704.115653

Gems D, Partridge L. Stress-response hormesis and aging: That which does not kill us makes us stronger. Cell Metabolism. 2008; 7(3): 200-203. doi: 10.1016/j.cmet.2008.01.001

Beal MF. Aging, energy and oxidative stress in neurodegenerative diseases. Ann Neurol. 1995; 38(3): 357-366. doi: 10.1002/ ana.410380304

Poulson HE, Preime H, Loft S. Role of oxidative DNA damage in cancer initiation and promotion. Eur J Cancer Prev. 1998; 7(1): 9-16

Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry. 1999; 64(4): 555-559. doi: 10.1016/S0308-8146(98)00102-2

Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology. 1999; 299: 152-178. doi: 10.1016/S0076-6879(99)99017-1

Oyaizu M. Studies on products of browning reaction prepared from glucosamine. Journal of Nutrition. 1986; 44: 307

Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite and nitrate in biological fluids. Anal Biochem. 1982; 126(1): 131-138. doi: 10.1016/0003- 2697(82)90118-X

Marcocci L, Packer L, Droy-Lefaix MT. Antioxidant action NC, et al 17 Adv Food Technol Nutr Sci Open J. 2019; 5(1): 13-18. doi: 10.17140/AFTNSOJ-5-152 of Ginkgo biloba extract EGB 761. Methods Enzymol. 1994; 234: 462-475. doi: 10.1016/0076-6879(94)34117-6

Tariq AL, Reyaz AL. Phytochemical analysis of Camellia sinensis leaves. International Journal of Drug Development and Research. 2012; 4(4): 311-316

Nor Qhairul Izzreen MN, Mohd Fadzelly AB. Phytochemicals and antioxidant properties of different parts of Camellia sinensis leaves from Sabah Tea Plantation in Sabah, Malaysia. International Food Research Journal. 2013; 20(1): 307-312

Oboh HA, Omoregie IP. Total phenolics and antioxidant capacity of some nigerian beverages. Nigerian Journal of Basic and Applied Scienc. 2011; 19(1): 68-75

Stankovic MS, Niciforovic N, Topuzovic M, Solujic S. Total phenolic content, flavonoid concentrations and antioxidant activity, of the whole plant and plant parts extracts from teucrium montanum L. var. montanum, F. supinum (L.) Reichenb. Biotechnology and Biotechnology. 2011; 25(1): 2222-2227. doi: 10.5504/ BBEQ.2011.0020

Ilesanmi FF, Akinloye OA, Ilesanmi OS. Comparative evaluation of in vitro antioxidant properties of cajanus cajan seed and Moringa oleifera leaf extracts. International Journal of Biochemistry Research and Review. 2014; 4(2): 163-172. doi: 10.9734/IJB-CRR/2014/6460

Aqil F, Ahmad I, Mehmood Z. Antioxidant and free radical scavenging properties of twelve traditionally used indian medicinal plants. Turkey Journal of Biology. 2006; 30: 177-183

Woranan K, Terdthai T, Jintanaporn W, Supaporn M, Panakaporn W, Jinatta J. Evaluation of total phenolic compound, antioxidant effect and neuropharmacological activities of Moringa oleifera lam. Leaves Extract. North-Eastern Thailand Journal of Neuroscience. 2012; 6(4): 80-92

Charoensin S. Antioxidant and anticancer activities of Moringa oleifera leaves. Journal of Medicinal Plant Research. 2014; 8(7): 318- 325. doi: 10.5897/JMPR2013.5353 Submit your article to this journal | https://openventio.org/submit-manuscript/

Koruthu DP, Manivarnan NK, Gopinath A, Abraham R. Antibacterial evaluation, reducing power assay and phytochemical screening of Moringa oleifera leaf extracts: Effect of solvent polarity. International Journal of Pharmaceutica. Sciences and Research. 2011; 2(11): 2991-2995

Nurul H, Masum KH, Abu H, Zulfiker KH, Kaniz FU. In vitro antioxidant activities of different parts of the plant Moringa oleifera lam. Research Journal Pharmarcy and Technology. 2012; 5(12): 1532-1537

Tariq AL, Reyaz AL. Antioxidant activity of Camellia sinensis leaves. Interational Journal of Current Microbiology and Applied Sciences. 2013; 2(5): 40-46

Ogbunugafor H, Igwo-Ezikpe M, Igwilo I, et al. In vitro and in vivo evaluation of antioxidant properties of Moringa oleifera ethanolic leaves extract and effect on serum lipid indices in rat. Macedonian Journal of Medical Sciences. 2012; 5(4): 397-403. doi: 10.3889/MJMS.1857-5773.2012.0240

Jagetia GC, Rao SK, Baliga MS, Babu KS. The Evaluation of nitric oxide scavenging activity of certain herbal formulations in vitro: A preliminary study. Phytother Res. 2004; 18(7): 561-565. doi: 10.1002/ptr.1494

Bryan NS, Grisham MB. Methods to detect nitric oxide and its metabolites in biological samples. Free Radic Biol Med. 2007; 43(5): 645-657. doi: 10.1016/j.freeradbiomed.2007.04.026

Kelm M. Nitric oxide metabolism and breakdown. Biochim Biophys Acta. 1999; 1411(2-3): 273-289. doi: 10.1016/S0005- 2728(99)00020-1

Sun J, Zhang X, Broderick M, Fein H. Measurement of nitric oxide production in biological systems by using Griess reaction assay. Sensors. 2003; 3(8): 276-284. doi: 10.3390/s30800276

Hausladen A, Stamler JS. Nitrosative stress. Methods Enzymol. 1999; 300: 389-395. doi: 10.1016/S0076-6879(99)00143-3

Published

2019-01-10

How to Cite

Moses Babatunde Ayoola, Nonso C. Ejiofor, & Ikechukwu Edwin Ezeagu. (2019). In Vitro-Evaluation of the Antioxidant Properties of Moringa Oleifera and Camelia Sinensis Leaves. Advances in Food Technology and Nutrition Sciences, 5(1), 13–18. https://doi.org/10.17140/aftnsoj-5-152